Journal: Journal of Nanobiotechnology

Article Title: Self-healing hybrid hydrogels with sustained bioactive components release for guided bone regeneration

doi: 10.1186/s12951-023-01811-8

Figure Lengend Snippet: BMSCs adhesion on the hydrogels and cells penetration analysis on electrospun membrane (n = 3). A Schematic illustration of the cell barrier function and cell behaviors in the stage of bone regeneration; B the confocal scanning of the control chambers, the 24-well plate, and the electrospun membrane after 1, 3 and 5 days, the scale bar is 200 μm; C confocal image and 3D reconstruction of the L929s cultured on the membrane surface after 5 days; D the BSA adsorption at 6 and 24 h of hydrogels and electrospun membrane; E the BMSCs morphology after cultured for 1, 3 and 5 days. The scale bar is 100 μm; F The live/dead staining of BMSCs cultured for 1, 3 and 5 days. The scale bar is 200 μm. BMSCs osteogenesis analysis in vitro . A The ALP staining and B activityon the 4th and the 7th day (n = 3); C the Alizarin red S staining was carried out on the 14th day; D the relative mRNA expressions of Runx2 and Alp in BMSCs cultured on different hydrogels for 4 and 7 days, while Ocn and Opn for 7 and 14 days (n = 3); E the Runx2 immunofluorescent staining of BMSCs cultured on different hydrogels for 4 days and 7 days and the Opn for 7 days and 14 days (n = 3). The scale bar is 200 μm. *** p < 0.001, ** p < 0.01, * p < 0.05

Article Snippet: The slices were stained with hematoxylin and eosin (H&E), Masson’s trichrome staining, and immunohistochemical (IHC) staining which incubated with primary antibodies against Runx2 (GB11264, ServiceBio, China), Col I (GB11022-3, ServiceBio, China), and Ocn (GB11233, ServiceBio, China), respectively.

Techniques: Cell Culture, Adsorption, Staining, In Vitro

Journal: Journal of Nanobiotechnology

Article Title: Self-healing hybrid hydrogels with sustained bioactive components release for guided bone regeneration

doi: 10.1186/s12951-023-01811-8

Figure Lengend Snippet: BMSCs osteogenesis analysis in vitro . A The ALP staining and B activityon the 4th and the 7th day (n = 3); C the Alizarin red S staining was carried out on the 14th day; D the relative mRNA expressions of Runx2 and Alp in BMSCs cultured on different hydrogels for 4 and 7 days, while Ocn and Opn for 7 and 14 days (n = 3); E the Runx2 immunofluorescent staining of BMSCs cultured on different hydrogels for 4 days and 7 days and the Opn for 7 days and 14 days (n = 3). The scale bar is 200 μm. *** p < 0.001, ** p < 0.01, * p < 0.05

Article Snippet: The slices were stained with hematoxylin and eosin (H&E), Masson’s trichrome staining, and immunohistochemical (IHC) staining which incubated with primary antibodies against Runx2 (GB11264, ServiceBio, China), Col I (GB11022-3, ServiceBio, China), and Ocn (GB11233, ServiceBio, China), respectively.

Techniques: In Vitro, Staining, Cell Culture

Journal: Journal of Nanobiotechnology

Article Title: Self-healing hybrid hydrogels with sustained bioactive components release for guided bone regeneration

doi: 10.1186/s12951-023-01811-8

Figure Lengend Snippet: Histological evaluation of bone regeneration and osteoblasts at 4 and 12 weeks in different groups. A The immunohistological staining of Runx2, Ocn, and Col I of newly generated bone tissue in the PLGA_30% nHA/DNH group, compared to the PLGA_PVGM/D group, the autogenous bone group and the untreated control (blank) at 4 and 12 weeks. The black dotted boxes in the upper panels were enlarged in the lower panels. The area marked by the dotted green line was new bone tissue, and the black triangle indicates positively stained cells (n = 5). The scale bar is 200 μm

Article Snippet: The slices were stained with hematoxylin and eosin (H&E), Masson’s trichrome staining, and immunohistochemical (IHC) staining which incubated with primary antibodies against Runx2 (GB11264, ServiceBio, China), Col I (GB11022-3, ServiceBio, China), and Ocn (GB11233, ServiceBio, China), respectively.

Techniques: Staining, Generated

Journal: International Journal of Molecular Sciences

Article Title: Chronic Partial Sleep Deprivation Increased the Incidence of Atrial Fibrillation by Promoting Pulmonary Vein and Atrial Arrhythmogenesis in a Rodent Model

doi: 10.3390/ijms25147619

Figure Lengend Snippet: Histological and Western blot analyses were performed on the atrial appendages of both left atriums (LAs) and right atriums (RAs) in both control and SD rats. ( A ) Masson staining of the atrial appendages in the LAs and RAs of both the control and SD groups highlighted a significant increase in fibrosis and collagen deposition in the SD LAs and RAs when compared to the corresponding structures in the control group. ( B ) Expression and phosphorylation levels of calcium-handling proteins, upstream kinases, and β1-adrenoceptor (β1-AR) in the control and SD LAs. ( C ) Expression and phosphorylation levels of calcium-handling proteins, upstream kinases, and β1-AR in the control and SD RAs. The expression level of G protein-coupled receptor kinase 2 (GRK2) was significantly reduced in the LAs of SD rats in comparison to those of control rats. β1-AR, beta-1 adrenoceptor; CaMKII, Ca 2+ /calmodulin-dependent kinase II-δ; pCaMKII, phosphorylated CaMKII at Thr286; NCX, Na + /Ca 2+ exchanger; Cav1.2, Cav1.2 L-type calcium channel; RyR2, ryanodine receptor type 2; pRyR S2808, phosphorylated RyR2 at Ser2808.

Article Snippet: All blots were probed with primary antibodies against a beta-1 adrenergic receptor (β1-AR, #PA1-049, Thermo Fisher Scientific, Waltham, MA, USA), G protein-coupled receptor kinase 2 (GRK2, #SC-562, Santa Cruz Biotechnology, Dallas, TX, USA), Ca 2+ /calmodulin-dependent kinase II-δ (CaMKII-δ, #GTX111401, GeneTex, Irvine, CA, USA), phosphorylated CaMKII at Thr 286 (pCaMKII, #ab32678, Abcam, Cambridge, UK), ryanodine receptor type 2 (RyR2, #MA3-916, Thermo Fisher Scientific, Waltham, MA, USA), phosphorylated RyR2 at Ser 2808 (pRyR S2808, #A010-30AP, Badrilla, Leeds, UK), catalytic subunit of protein kinase A (PKAc, #610981, BD Transduction Laboratories, San Jose, CA, USA), Na + /Ca 2+ exchanger (NCX, #R3F1, Swant, Burgdorf, Switzerland), Cav1.2 L-type calcium channel (Cav1.2, #ACC-003, Alomone Lab, Jerusalem, Israel), and glyceraldehyde-3-phosphate dehydrogenase (GADPH, #M171-7, MBL, Nagoya, Japan).

Techniques: Western Blot, Control, Staining, Expressing, Phospho-proteomics, Comparison

Journal: bioRxiv

Article Title: Nonlethal effects of predation: Presence of insectivorous birds affects the behaviour and level of stress in insects

doi: 10.1101/2022.04.01.486535

Figure Lengend Snippet: Effect of predator treatments on the Schgr-AKH-II level in CNS (a) and in haemolymph (b) of S. gregaria within the laboratory experiments. Statistically differences between the groups were evaluated using one-way ANOVA with Tukey’s posttest, and significant results are marked by *** P <0.001, * P <0.05, ns P >0.05. Treatments: Bird = real great tits were present in the experimental cages and warning calls were played, Call = only warning call of great tit was played, Control = only locusts were present in the cages.

Article Snippet: The primary rabbit antibodies used in the procedure (dilution 1:1000) were raised commercially against the Schgr-AKH-II by Moravian-Biotechnology (Brno, Czech Republic); to exclude a possible cross-reactivity, the corresponding pre-immune serum was used as well.

Techniques:

Journal: bioRxiv

Article Title: Nonlethal effects of predation: Presence of insectivorous birds affects the behaviour and level of stress in insects

doi: 10.1101/2022.04.01.486535

Figure Lengend Snippet: Effect of predator treatments on the Schgr-AKH-II level in CNS (a) and in haemolymph (b) of S. gregaria in the outdoor aviary experiments. Statistically significant differences between the groups were evaluated using the Student’s t -test, and significant results are marked by * P < 0.05, ns P > 0.05 ( n = 4-16). Treatments: Bird = real great tits were present in the aviary and warning calls were played, Control = only locusts were present in the nearby shady area without birds.

Article Snippet: The primary rabbit antibodies used in the procedure (dilution 1:1000) were raised commercially against the Schgr-AKH-II by Moravian-Biotechnology (Brno, Czech Republic); to exclude a possible cross-reactivity, the corresponding pre-immune serum was used as well.

Techniques: