Journal: BioMed Research International

Article Title: Restricted Water Intake and Hydration with Fructose-Containing Beverages during Infancy Predispose to Aggravate an Acute Renal Ischemic Insult in Adolescent Rats

doi: 10.1155/2020/4281802

Figure Lengend Snippet: Various parameters at the end of the 6 wks of follow-up evaluated preacute kidney injury (AKI) by ischemia/reperfusion: (a) urinary osmolality; (b) plasma copeptin; (c) creatinine clearance (CrCl); (d) nitrate/nitrite concentration (NO 2 /NO 3 ) in plasma; (e) nitrate/nitrite concentration (NO 2 /NO 3 ) in urine; (f) fructokinase (KHK) renal cortex expression; (g) angiotensin-converting enzyme 2 renal cortex expression; (h) angiotensin II renal cortex expression; (i) angiotensin II receptor type 1 (AT1-R) renal cortex expression; (j) kidney injury molecule 1 (KIM-1) renal cortex expression; (k) neutrophil gelatinase-associated lipocalin (NGAL) renal cortex expression; (l) lipid peroxidation measured in renal cortex homogenates. C: control; +W: increased water intake; +SB: sweetened beverage (SB) ad libitum; -W: fluid restriction plus hydration with tap water; -SB: fluid restriction plus hydration with SB. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

Article Snippet: Antibodies against kidney injury molecule 1 (KIM-1, Cat. GTX85067, dilution 1 : 2500), fructokinase (KHK, Cat. GTX109591, dilution 1 : 5000), angiotensin-converting enzyme 2 (ACE2, Cat. GTX101395, dilution 1 : 1500), and angiotensin II (Cat. GTX37789, dilution 1 : 1000) were obtained from GeneTex, Inc., Irvine, CA, USA.

Techniques: Clinical Proteomics, Concentration Assay, Expressing, Control

Journal: bioRxiv

Article Title: Nonlethal effects of predation: Presence of insectivorous birds affects the behaviour and level of stress in insects

doi: 10.1101/2022.04.01.486535

Figure Lengend Snippet: Effect of predator treatments on the Schgr-AKH-II level in CNS (a) and in haemolymph (b) of S. gregaria within the laboratory experiments. Statistically differences between the groups were evaluated using one-way ANOVA with Tukey’s posttest, and significant results are marked by *** P <0.001, * P <0.05, ns P >0.05. Treatments: Bird = real great tits were present in the experimental cages and warning calls were played, Call = only warning call of great tit was played, Control = only locusts were present in the cages.

Article Snippet: The primary rabbit antibodies used in the procedure (dilution 1:1000) were raised commercially against the Schgr-AKH-II by Moravian-Biotechnology (Brno, Czech Republic); to exclude a possible cross-reactivity, the corresponding pre-immune serum was used as well.

Techniques:

Journal: bioRxiv

Article Title: Nonlethal effects of predation: Presence of insectivorous birds affects the behaviour and level of stress in insects

doi: 10.1101/2022.04.01.486535

Figure Lengend Snippet: Effect of predator treatments on the Schgr-AKH-II level in CNS (a) and in haemolymph (b) of S. gregaria in the outdoor aviary experiments. Statistically significant differences between the groups were evaluated using the Student’s t -test, and significant results are marked by * P < 0.05, ns P > 0.05 ( n = 4-16). Treatments: Bird = real great tits were present in the aviary and warning calls were played, Control = only locusts were present in the nearby shady area without birds.

Article Snippet: The primary rabbit antibodies used in the procedure (dilution 1:1000) were raised commercially against the Schgr-AKH-II by Moravian-Biotechnology (Brno, Czech Republic); to exclude a possible cross-reactivity, the corresponding pre-immune serum was used as well.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Chronic Partial Sleep Deprivation Increased the Incidence of Atrial Fibrillation by Promoting Pulmonary Vein and Atrial Arrhythmogenesis in a Rodent Model

doi: 10.3390/ijms25147619

Figure Lengend Snippet: Histological and Western blot analyses were performed on the atrial appendages of both left atriums (LAs) and right atriums (RAs) in both control and SD rats. ( A ) Masson staining of the atrial appendages in the LAs and RAs of both the control and SD groups highlighted a significant increase in fibrosis and collagen deposition in the SD LAs and RAs when compared to the corresponding structures in the control group. ( B ) Expression and phosphorylation levels of calcium-handling proteins, upstream kinases, and β1-adrenoceptor (β1-AR) in the control and SD LAs. ( C ) Expression and phosphorylation levels of calcium-handling proteins, upstream kinases, and β1-AR in the control and SD RAs. The expression level of G protein-coupled receptor kinase 2 (GRK2) was significantly reduced in the LAs of SD rats in comparison to those of control rats. β1-AR, beta-1 adrenoceptor; CaMKII, Ca 2+ /calmodulin-dependent kinase II-δ; pCaMKII, phosphorylated CaMKII at Thr286; NCX, Na + /Ca 2+ exchanger; Cav1.2, Cav1.2 L-type calcium channel; RyR2, ryanodine receptor type 2; pRyR S2808, phosphorylated RyR2 at Ser2808.

Article Snippet: All blots were probed with primary antibodies against a beta-1 adrenergic receptor (β1-AR, #PA1-049, Thermo Fisher Scientific, Waltham, MA, USA), G protein-coupled receptor kinase 2 (GRK2, #SC-562, Santa Cruz Biotechnology, Dallas, TX, USA), Ca 2+ /calmodulin-dependent kinase II-δ (CaMKII-δ, #GTX111401, GeneTex, Irvine, CA, USA), phosphorylated CaMKII at Thr 286 (pCaMKII, #ab32678, Abcam, Cambridge, UK), ryanodine receptor type 2 (RyR2, #MA3-916, Thermo Fisher Scientific, Waltham, MA, USA), phosphorylated RyR2 at Ser 2808 (pRyR S2808, #A010-30AP, Badrilla, Leeds, UK), catalytic subunit of protein kinase A (PKAc, #610981, BD Transduction Laboratories, San Jose, CA, USA), Na + /Ca 2+ exchanger (NCX, #R3F1, Swant, Burgdorf, Switzerland), Cav1.2 L-type calcium channel (Cav1.2, #ACC-003, Alomone Lab, Jerusalem, Israel), and glyceraldehyde-3-phosphate dehydrogenase (GADPH, #M171-7, MBL, Nagoya, Japan).

Techniques: Western Blot, Control, Staining, Expressing, Phospho-proteomics, Comparison